Plotting

OligoMacro Toolset, is an ImageJ macro-toolset aimed at isolating oligodendrocytes from wide-field images, tracking isolated cells, characterizing processes morphology along time, outputting numerical data and plotting them. It takes benefit of ImageJ built-in functions to process images and extract data, and relies on the R software in order to generate graphs.

shinyHTM is an open source, web-based tool for data exploration, image visualization and normalization of High Throughput Microscopy data. Within shinyHTM the user is guided through a linear workflow which follows the following best practices:

• Inspect the numerical data through plotting
• Measurements are linked to raw images
• Perform quality control to exclude images with aberrations or where image analysis failed
• Perform a reproducible data analysis
• Normalize data and report statistical significance

Image visualization relies on Fiji/ImageJ, along with its wealth of analytical tools.

shinyHTM can be used to analyze image features obtained with CellProfiler, ImageJ or any other bioimage analysis software. The output of analysis is a publication-ready scoring of the data.

shinyHTM is based on the R shiny package.

Python is a programming language.

Python 2.7.0 was released on July 3rd, 2010.

Python 2.7 is scheduled to be the last major version in the 2.x series before it moves into an extended maintenance period. This release contains many of the features that were first released in Python 3.1.

 A bugfix release, 2.7.16, is currently available. Its use is recommended.