Manual

Description

This toolkit extracts Spherical Textures: Angular projections of 2D or 3D image objects with subsequent spherical harmonics analysis.

From the author summary: "We introduce a novel method to extract quantitative data from microscopy images by precisely measuring the distribution of intensities within objects in both 3D and 2D. This method is easily accessible through the object classification workflow of ilastik, provided the original image is segmented into separate objects. The method is specifically designed to analyze the convex region in objects, focusing on the variation in fluorescence intensity caused by differences in their shapes or patterns."

Fig. 2 from reference publication
Description

napari-lattice is a napari plugin designed for the analysis and visualization of Lattice Lightsheet Microscopy (LLSM) and Oblique Plane Microscopy (OPM) data, particularly focusing on data acquired from Zeiss Lattice Lightsheet systems. Also available as lls-core - a command line version of the same tool which does not require napari.

napari-lattice allows users to deskew and deconlolve lattice light sheet, or any oblique plane microscopy, data. To speed processing, users can provide ROIs to be cropped and processed separately.  This significantly speeds up processing time and allows many options for parallelisation. 

Description

AnyLabeling is Effortless AI-assisted data labeling tool with AI support from Segment Anything and YOLO models!

AnyLabeling = LabelImg + Labelme + Improved UI + Auto-labeling

Installation

Standalone (executable)

The executable file links are provided in Assets section here

Install from source

git clone https://github.com/vietanhdev/anylabeling
cd anylabeling
pip install .

Install from PyPI

pip install anylabeling

With GPU support:

pip install anylabeling-gpu
Description

BraiAn is an open-source suite of tools designed to simplify signal quantification, analysis and visualization of large datasets typically obtained in whole-brain imaging experiments, following registration to an atlas. 

The package consists of two separate modules.

  1. BrainAnDetect: A QuPath extension for multi-channel cell segmentation across large and variable datasets. It leverages QuPath's built in algorithms for cell detection, and features additional options for refining signal quantification, including machine-learning-based object classification, region-specific cell segmentation, multiple marker co-expression analysis, and an interface for selective exclusion of damaged tissue portions.
  2. BraiAnalyse: A modular Python library for the easy navigation, visualization, and analysis of whole-brain quantification outputs.
has topic
need a thumbnail
Description

MetaXpress or in full name "MetaXpress® High-Content Image Acquisition and Analysis Software" is a commercially available closed source software for high-content analysis from Molecular Devices, LLC.. The program is a kind of visually guided workflow programming environment. There is a programming module called CME (custom module editor) which lets one setup integrated workflows for bioimage analysis with visual feedback. It is designed for high-throughput in connection with a included database which stores the experimental data. 

It has several toolboxes for semiautomated processing of various tasks:

3D Analysis (requires Custom Module Editor), Curve fitting, Transmitted light segmentation (requires Custom Module Editors), Angiogenesis tube formation, Cell cycle, Cell health, Cell scoring , Count nuclei, Granularity, Live/dead , Mitotic index, Micronuclei , Monopole detection, Multi-Wavelength cell scoring, Multi-wavelength translocation, Neurite outgrowth , Transfluor® Assay, Translocation* (includes Translocation-Enhanced*) , Transfluor HT Assay , Nuclear translocation HAT, Cell proliferation HT

After the workflow is setup it is possible to apply it automatically to a stack of stored images. The derived data from those analyses is stored in the metaxpress database and can be exported from there.

The use of each toolbox requires a separate license.