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A generalist framework for multi-dimensional automatic spot detection and quantification.

SpotMAX is designed to accomplish two tasks:

1. Detecting and quantifying globular-like structures (a.k.a. "spots")
2. Segmenting and quantifying fluorescently labelled structures

It supports 2D, 3D, 4D, and 5D data, i.e., z-stacks, timelapse, and multiple fluorescence channels (and combinations thereof).

This workflow is the integration of YOLO (You Only Look Once) machine learning models, image pre-processing scripts and labeling tools within the Galaxy platform. Galaxy is an open, web-based platform used primarily for data analysis in computational biology, but it also has applications in image processing and other fields. 

How the Galaxy YOLO image segmentation tool works

The combination of Galaxy and YOLO allows researchers to perform object detection and image analysis without requiring extensive programming knowledge. Here's how it generally works: 

• Web-based interface: Galaxy provides a graphical, user-friendly interface to access powerful analysis tools. Users can simply upload their image data, select the YOLO tool, and run the analysis.
• YOLO model execution: The Galaxy tool executes a pre-trained YOLO model, often from the Ultralytics framework, on the input images. These models can perform tasks like object detection (drawing bounding boxes) or instance segmentation (creating pixel-level masks).
• Training and prediction: Some tools allow for both model training and prediction. Users can train a custom YOLO model on their own labeled datasets to detect specific objects of interest. For example, bioimage analysis may involve detecting cells or other structures.
• Other integrations: Other machine-learning tools can be integrated with YOLO in Galaxy. For instance, the AnyLabeling tool supports YOLO for semi-automated and active learning-based data annotation. 

Description from Github page:

A GUI-based Python framework for segmentation, tracking, cell cycle annotations and quantification of microscopy data.
Provides a GUI for neural network models including Segment Anything Model (SAM), YeaZ, cellpose, StarDist, YeastMate, omnipose, delta, DeepSea.

# Install the ultralytics package from PyPI
pip install ultralytics

You can also install ultralytics directly from the Ultralytics GitHub repository. This can be useful if you want the latest development version. Ensure you have the Git command-line tool installed, and then run:

# Install the ultralytics package from GitHub
pip install git+https://github.com/ultralytics/ultralytics.git@main

Aligning Big Brains & Atlases (ABBA) is a set of software components which allows users to register images of thin serial biological tissue sections, cut in any orientation (coronal, sagittal or horizontal) to atlases, usually brain atlases. ABBA is available as a Fiji plugin for performing registration; a QuPath extension is also available and recommended. Typically, a set of serial sections is defined as a QuPath project, that is registered within Fiji. The registration results can then imported back into QuPath for downstream processing (cell detection and classification, cell counting per region, etc.).

Available atlases include the 3D mouse Allen Brain atlas and the Waxholm Space Atlas of the Sprague Dawley Rat Brain. Depending on your installation method, you may also access all BrainGlobe atlases.