Image processing

Synonyms
Image pre-processing
Image filtering
Image preprocessing
Image postprocessing
Image post-processing
Image manipulation

GPU-Accelerating ImageJ Macro Image Processing Workflows Using CLIJ

Submitted by czhang on Thu, 04/27/2023 - 09:55

This chapter is part of this book. The chapter introduces GPU-accelerated image processing in ImageJ/Fiji. The reader is expected to have some pre-existing knowledge of ImageJ Macro programming. Core concepts such as variables, for-loops, and functions are essential. The chapter provides basic guidelines for improved performance in typical image processing workflows.

Description

Removal of heterogeneous background from image data of single-molecule localization microscopy, using extreme value-based emitter recovery (EVER).

Quote:

EVER requires no manual adjustment of parameters and has been implemented as an easy-to-use ImageJ plugin that can immediately enhance the quality of reconstructed super-resolution images. This method is validated as an efficient way for robust nanoscale imaging of samples with heterogeneous background fluorescence, such as thicker tissue and cells.

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